FIGURE

Fig. 3

ID

ZDB-FIG-131015-5

Publication

Kwong et al., 2013 - The Role of Aquaporin and Tight Junction Proteins in the Regulation of Water Movement in Larval Zebrafish (Danio rerio)

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Fig. 3

Aquaporin-1a1 is expressed in ionocytes on the skin of yolk sac in larval zebrafish.

(A) A representative western blot showing that the eel aquaporin-1a1 (AQP1a1) antibody yielded a single immunoreactive band at ~27 kDa in lysates of 4 dpf zebrafish larvae. (B) Fluorescent immunohistochemistry and confocal microscopy revealed that the expression of AQP1a1 on the skin of yolk sac (green), and a subset of cells stained positively with Mitotracker^® (red) and concanavalin-A (conA; blue). (D) is the merged images of B and C. Cells labelled with arrowheads represent areas of colocalization of AQP1a1 and Mitotracker^® or conA. (E) Some AQP1a1-positive cells (green) were also colocalized with (F) Na⁺/K⁺-ATPase staining (NKA; red). (G) is the merged images of E and F. Cells labelled with arrows represent areas of colocalization of AQP1a1 and NKA. Scale bar = 25 μm.

Expression Data

Antibodies:	Ab1-aqp1a.1 Ab1-atp1a
Fish:	WT
Anatomical Term:	ball integument ionocyte
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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