FIGURE

Fig. S1

ID

ZDB-FIG-130930-10

Publication

Lin et al., 2013 - Low temperature mitigates cardia bifida in zebrafish embryos

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Fig. S1

Genetic mapping of the s1pr2^as10 mutant gene. (A) Meiotic map of the s1pr2^as10 locus. The numbers above the line indicate the number of meiotic recombination events out of the total number of meioses. (B) An enlarged view of the mil/edg5 locus shows the insertion in intron 1. Three primers (F1, R1, and I1) were used to confirm the insertion. (C) A 182-bp DNA fragment amplified using primers F1 and R1 was detected in wild-type (WT) and s1pr2^as10 heterozygous mutants, whereas a 426-bp DNA fragment amplified using primers I1 and R1 was detected in s1pr2^as10 heterozygous and homozygous mutant embryos. (D) Expression of mil/edg5 mRNA was reduced in s1pr2^as10 mutants prior to 96 hpf, as revealed by qRT-PCR. WT (+, W); s1pr2^as10 mutant (, M). The error bars indicate the standard error.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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