ZFIN ID: ZDB-FIG-130906-47
Czopka et al., 2013 - Individual Oligodendrocytes Have Only a Few Hours in which to Generate New Myelin Sheaths In Vivo. Developmental Cell   25(6):599-609 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Protruding-mouth to Days 7-13

Fig. 6

Myelin Sheaths Are Generated during the Same Short Dynamic Period following Fyn Loss and Gain of Function (A) Images of a single oligodendrocyte in a Tg(nkx2.2a:mEGFP) fyn morphant during initiation of myelination. The first ensheathment is indicated with “#1.” Scale bar, 10 μm. (B) Images of a single oligodendrocyte in a Tg(nkx2.2a:mEGFP), Tg(mbp:ca-Fyn) animal during initiation of myelination. The first ensheathments are indicated with “#1” and “#2.” Scale bar, 10 μm. (C) Total number of nascent myelin sheaths for 13 different fyn morphant oligodendrocytes over time. Time point zero is set as the time at which each oligodendrocyte starts to make its first myelin sheath. The cell from (A) is colored in cyan. (D) Total number of nascent myelin sheaths for 14 different oligodendrocytes in Tg(mbp:ca-Fyn) animals over time. Time-point zero is set as the time at which each oligodendrocyte starts to make its first myelin sheath. The cell from (B) is colored in cyan. (E) Ensheathment, but not retraction, number changes with Fyn manipulation. Significance was assessed using two-way ANOVA. Error bars indicate SD. (F) The duration of myelin sheath formation by individual oligodendrocytes is similar in wild-type, fyn morphant, and Tg(mbp:ca-Fyn) animals. Significance was assessed using one-way ANOVA. Error bars indicate SD. See also Figures S1 and S2 and Movies S1, S2, and S3.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ue5Tg; vu16Tg standard conditions Protruding-mouth to Days 7-13 oligodendrocyte IFL
Protruding-mouth to Days 7-13 oligodendrocyte myelin sheath IFL
vu16Tg + MO1-fyna,fynb standard conditions Protruding-mouth to Days 7-13 oligodendrocyte IFL
Protruding-mouth to Days 7-13 oligodendrocyte myelin sheath IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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Reprinted from Developmental Cell, 25(6), Czopka, T., Ffrench-Constant, C., and Lyons, D.A., Individual Oligodendrocytes Have Only a Few Hours in which to Generate New Myelin Sheaths In Vivo, 599-609, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell