ZFIN Figure: Czopka et al., 2013, Fig. 6

ZFIN ID: ZDB-FIG-130906-47

ZFIN ID: ZDB-FIG-130906-47

Czopka et al., 2013 - Individual Oligodendrocytes Have Only a Few Hours in which to Generate New Myelin Sheaths In Vivo. Developmental Cell 25(6):599-609 Full text @ Dev. Cell

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Gene:	EGFP
Fish:	ue5Tg; vu16Tg vu16Tg + MO1-fyna,fynb
Knockdown Reagent:	MO1-fyna,fynb
Anatomical Terms:	oligodendrocyte oligodendrocyte myelin sheath
Stage Range:	Protruding-mouth to Days 7-13

Fig. 6

Myelin Sheaths Are Generated during the Same Short Dynamic Period following Fyn Loss and Gain of Function (A) Images of a single oligodendrocyte in a Tg(nkx2.2a:mEGFP) fyn morphant during initiation of myelination. The first ensheathment is indicated with “#1.” Scale bar, 10 μm. (B) Images of a single oligodendrocyte in a Tg(nkx2.2a:mEGFP), Tg(mbp:ca-Fyn) animal during initiation of myelination. The first ensheathments are indicated with “#1” and “#2.” Scale bar, 10 μm. (C) Total number of nascent myelin sheaths for 13 different fyn morphant oligodendrocytes over time. Time point zero is set as the time at which each oligodendrocyte starts to make its first myelin sheath. The cell from (A) is colored in cyan. (D) Total number of nascent myelin sheaths for 14 different oligodendrocytes in Tg(mbp:ca-Fyn) animals over time. Time-point zero is set as the time at which each oligodendrocyte starts to make its first myelin sheath. The cell from (B) is colored in cyan. (E) Ensheathment, but not retraction, number changes with Fyn manipulation. Significance was assessed using two-way ANOVA. Error bars indicate SD. (F) The duration of myelin sheath formation by individual oligodendrocytes is similar in wild-type, fyn morphant, and Tg(mbp:ca-Fyn) animals. Significance was assessed using one-way ANOVA. Error bars indicate SD. See also Figures S1 and S2 and Movies S1, S2, and S3.

Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
EGFP	ue5Tg; vu16Tg	standard conditions	Protruding-mouth to Days 7-13	oligodendrocyte	IFL
			Protruding-mouth to Days 7-13	oligodendrocyte myelin sheath	IFL
	vu16Tg + MO1-fyna,fynb	standard conditions	Protruding-mouth to Days 7-13	oligodendrocyte	IFL
			Protruding-mouth to Days 7-13	oligodendrocyte myelin sheath	IFL

Antibody Labeling Details No data available

Phenotype Details No data available

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Reprinted from Developmental Cell, 25(6), Czopka, T., Ffrench-Constant, C., and Lyons, D.A., Individual Oligodendrocytes Have Only a Few Hours in which to Generate New Myelin Sheaths In Vivo, 599-609, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell

Acknowledgments:

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 25(6), Czopka, T., Ffrench-Constant, C., and Lyons, D.A., Individual Oligodendrocytes Have Only a Few Hours in which to Generate New Myelin Sheaths In Vivo, 599-609, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell