FIGURE

Fig. S2

ID
ZDB-FIG-130808-8
Publication
Laranjeiro et al., 2013 - Cyclin-dependent kinase inhibitor p20 controls circadian cell-cycle timing
Other Figures
All Figure Page
Back to All Figure Page
Fig. S2

Rev-Erbα binds to the RRE in the p20 promoter. (A) Western blot analysis of BL21 bacterial cells expressing either GST or GST-HA-mRev-erbα using an anti-HA antibody. (B) Electrophoretic mobility shift assay using a 32P-labeled DNA fragment of the p20 promoter containing (p20 WT probe) or lacking (p20 ΔRRE probe) the RRE sequence. Arrow indicates a specific shift observed only for the p20 WT probe in the presence of Rev-Erbα. Unlabeled oligo containing the p20 RRE competes with binding to the labeled probe.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA