Fig. S2
- ID
- ZDB-FIG-130808-8
- Publication
- Laranjeiro et al., 2013 - Cyclin-dependent kinase inhibitor p20 controls circadian cell-cycle timing
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Rev-Erbα binds to the RRE in the p20 promoter. (A) Western blot analysis of BL21 bacterial cells expressing either GST or GST-HA-mRev-erbα using an anti-HA antibody. (B) Electrophoretic mobility shift assay using a 32P-labeled DNA fragment of the p20 promoter containing (p20 WT probe) or lacking (p20 ΔRRE probe) the RRE sequence. Arrow indicates a specific shift observed only for the p20 WT probe in the presence of Rev-Erbα. Unlabeled oligo containing the p20 RRE competes with binding to the labeled probe. |