FIGURE

Fig. 2

ID

ZDB-FIG-130801-40

Publication

Bohni et al., 2013 - Integration of Microfractionation, qNMR and Zebrafish Screening for the In Vivo Bioassay-Guided Isolation and Quantitative Bioactivity Analysis of Natural Products

Other Figures

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Fig. 2

Anti-angiogenic activity of the methanolic extract of Rhynchosia viscosa.

Inhibition of vascular outgrowth was determined in fli-1:EGFP transgenic embryos. At 16 hours post-fertilization (hpf), embryos were incubated with different concentrations of the methanolic extract of the plant and anti-angiogenic effects were assessed at 48 hpf. A to C, all embryos are 48 hpf, with anterior to the left, scale bar = 10 μm. A, untreated control (DMSO 1%); B, zoom of A (dashed box) showing normal outgrowth of intersegmental vessels (ISV) along the trunk of the larva (arrows); C, embryo treated with 50 μg/mL crude methanolic extract of R. viscosa. Inhibition or reduction of ISV growth is observed along the trunk (arrows); D, IC₅₀ curve and values showing the inhibitory activity of the methanolic extract of R. viscosa.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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