Fig. S7

Abnormal PCP does not disrupt Wnt/β-catenin signaling or posterior tissue fate specification. (A) qRT-PCR to analyze expression of Wnt/β-catenin target genes in vangl2 mutants relative to wild-type (WT) reveals no difference in expression levels of axin2 (P=0.6314, Student’s t-test), lef1 (P=0.3469, Student’s t-test), or cyclin D1 (P=0.8825, Student’s t-test). Error bars represent standard error of the fold change. Each graph is representative of two independent experiments with three technical replicates each. (B) Lateral views of whole-mount in situ hybridization for the posterior mesodermal marker tbx6 in wild-type/vangl2^+/– and vangl2 mutant embryos. (C) Quantification of the size of the tbx6 expression domain relative to the total embryonic body length in WT and vangl2 mutant embryos. The tbx6 expression domain is not significantly expanded in vangl2 mutant embryos. n.s., not significant, Students t-test.