FIGURE

Fig. s1

ID

ZDB-FIG-130326-59

Publication

Fraser et al., 2013 - Regeneration of cone photoreceptors when cell ablation is primarily restricted to a particular cone subtype

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Fig. s1

Assessing the quality of transgene expression in photoreceptors. We assessed our novel transgenic driver line, intended to express Gal4-VP16 in UV cone photoreceptors, by breeding it to two reporter lines. One reporter, primarily used in our experiments, drives expression of nfsB-mCherry (NTR-mCherry protein), and the other reporter is GFP. Thus breeding created Tg(SWS1:Gal4-VP16)ua3016;Tg(UAS-E1b:NfsB-mCherry)c264;Tg(4xUAS:GFP)hzm3 zebrafish. The inset, from a fish at 22 days post-fertilization (dpf), is magnified at the bottom of the figure and is consistent with data from fish at 42 or 66 dpf, regarding GFP being present in more cones than mCherry. Thus we cannot rule out deficits with the Gal4-VP16 driver line as contributing to the lack of robust NTR-mCherry expression in all UV cones. Further, the quantity of nfsb-mCherry expressing cells is substantially decreased in older fish.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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