FIGURE

Fig. 4

ID

ZDB-FIG-130228-31

Publication

Mikelsaar et al., 2012 - Epitope of titin a-band-specific monoclonal antibody Tit1 5 H1.1 is highly conserved in several Fn3 domains of the titin molecule. Centriole staining in human, mouse and zebrafish cells

Other Figures

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Fig. 4

Immunofluorescence co-localization of MAb Tit1 5 H1.1 target antigen (titin) with F-actin in different cells of zebrafish primary culture of the testes. Cells were fixed on 3rd day of cultivation with 4% PFA, permeabilized, and double-labelled (C) with Mab Tit1 5 H1.1 for titin (red - Alexa 594) and with Phalloidin for F-actin (green – Alexa 488). Cell nuclei were stained blue with DAPI (obj. 100x). Arrows show centriole staining.In Photoshop image processing either red colour (A) or green colour (B) was removed and one can see independent staining both of F-actin (A) and titin (B). Note a very extensive fibrous centriole-orientated staining of titin.

Expression Data

Gene:	ttn.2
Antibody:	Ab2-ttn
Fish:	WT
Anatomical Terms:	testis centriole testis cytoplasm testis nucleus
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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