Fig. 3
Combinatorial injections of fzd3a, fzd9b and fzd10–MO do not affect melanisation. A–F) 1 dpf embryos. Typical melanisation patterns of embryos showing “elongated axis+no/slight necrosis” phenotype in Table 3 are presented here for each injection. G–L) 3 dpf embryos. All are left side views with dorsal oriented to the top. A, G) Control standard-MO, 17 ng/embryo. B, H) fzd3a, fzd9b, fzd10-MO = 10, 5, 2, ng/embryos, respectively. C, I) fzd3a, fzd9b, fzd10-MO = 10, 5, 1, ng/embryos, respectively. D, J) fzd3a, fzd9b, fzd10-MO = 10, 2, 2, ng/embryos, respectively. E, K) fzd3a, fzd9b, fzd10-MO = 10, 2, 1, ng/embryos, respectively. F, L) fzd3a, fzd9b, fzd10-MO = 10, 1, 1, ng/embryos, respectively. Scale bars: 200 μm. |
Fish: | |
---|---|
Knockdown Reagents: | |
Observed In: | |
Stage Range: | Prim-5 to Protruding-mouth |