Fig. S12
The mesenchymal cells are polarized. A Representative immunostaining with anti-GFP and anti-γTubulin antibodies in 2 dpf uncut transgenic osteopontin:eGFP larvae (single frame). B–C Representative immunostaining with anti-GFP anti-γTubulin antibodies in amputated transgenic osteopontin:eGFP larvae of 2 dpf 1 hpa and 3 dpf 1 dpa (single frames). A′–C′ Representative single frames of the corresponding zoomed area represented by a square in A–C. Merged and single color images of the MTOC (γTubulin, red) together with the nuclei (DAPI, blue) and osteopontin:eGFP labeling the mesenchymal cells (anti-GFP, green), respectively. The arrows highlight the presence of a MTOC to allow better comparison of its position relative to the corresponding nucleus. 5 Larvae per condition. Scale bar corresponds to 50 μm in all images. |