FIGURE

Fig. 3

ID

ZDB-FIG-130117-13

Publication

Lagendijk et al., 2012 - Revealing details: whole mount microRNA in situ hybridization protocol for zebrafish embryos and adult tissues

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Fig. 3

Whole mount miRNA in situ hybridization on adult tissues.

(A–D) MiR-23 is expressed in the bulbus arteriosus (b) of zebrafish adult hearts fixed in PFA (A,C) and PFA+EDC (B–D). PFA+EDC fixed hearts revealed a more detailed miR-23 expression pattern with a ring-like expression (arrowhead in B) at the inflow area of the atrium (a) and in coronary arteries (arrowheads in D) overlying the ventricle (v). (E,F) Robust miR-23 expression was detected in joint structures of PFA+EDC fixed caudal fins (arrowheads in F) versus more diffuse miR-23 expression in caudal fins fixed in PFA only (E). (G) Negative control heart was fixed in PFA+EDC but not incubated with a probe. Scale bars represent 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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