FIGURE

Fig. 2

ID

ZDB-FIG-120914-4

Publication

Song et al., 2012 - Effective gene trapping mediated by sleeping beauty transposon

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Fig. 2

Activity of the trapping cassette in an intron of pSPL3 vector.

The trapping cassette was subcloned into the BamHI/EcoRI site of pSPL3 vector to generate the pSPL3-Trap(intron), which was used for transient transfection of HeLa cells at 80% confluence. Images were taken under a Nikon TE2000 fluorescent microscope at 48 h after transfection and cell numbers from three independent transfections were counted. Zebrafish embryos at one-cell stage were microinjected with the pSPL3-Trap(intron). Injected embryos at 24 hpf were imaged under a SteReo Lumar V12 microscope form Zeiss and total embryos in three dishes were counted. The ectopic expression of EGFP in one embryo was enlarged and shown in a merged image. SD1, splice donor for exon1; IR/DR(L) and IR/DR(R), left and right inverted repeat/directed repeat of the SB transposon; SA, splice acceptor; IRES, internal ribosome entry site; EGFP, enhanced green fluorescence protein gene; poly(A), poly(A) signal; SA2, splice acceptor for exon2.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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