FIGURE

Fig. 4

ID

ZDB-FIG-120831-12

Publication

Eom et al., 2012 - Melanophore Migration and Survival during Zebrafish Adult Pigment Stripe Development Require the Immunoglobulin Superfamily Adhesion Molecule Igsf11

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Fig. 4

seurat mutant phenotype rescued by wild-type igsf11 expressed in mitfa+ pigment cell precursors.

(A) Juvenile seurat mutant. (B) Sibling of fish in A injected with mitfa:nlsVenus-V2a-igsf11 transgene at the one cell stage exhibits patches of rescued melanophore stripes (arrowheads). (C,C2) Close-up showing individual melanophores (e.g., arrowhead) that express nlsVenus. This individual had been treated with epinephrine to contract melanin-containing organelles (melanosomes) towards the cell body, which facilitates fluorophore visualization; melanosomes of only some melanophores are in the fully contracted state. Arrow, xanthophores autofluorescence in the same channel as nlsVenus. (D) Adult wild-type. (E) Adult seurat mutant. (F, G) Two examples of seurat mutants transgenic for stably integrated mitfa:igsf11, which exhibit nearly wild-type stripes marked by occasional breaks (arrows). Individuals were genotyped by PCR to verify presence of the transgene. Scale bar: in (C) 60 μm for (C,C2).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	igsf11^{utr15e1/utr15e1} (AB) igsf11^{utr15e1/utr15e1}; ou2014Tg
Observed In:	melanophore stripe
Stage Range:	Days 45-89 to Adult

Phenotype Detail

Acknowledgments

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