ZFIN ID: ZDB-FIG-120724-4
Akle et al., 2012 - F-Spondin/spon1b Expression Patterns in Developing and Adult Zebrafish. PLoS One   7(6):e37593 Full text @ PLoS One
Anatomical Terms:
Stage Range: Protruding-mouth to Adult

Fig. 4 Spon1b:GFP expression in the habenular nuclei and their projections.

A-C. Dorsal view of live zebrafish showing migration of ventral habenular nuclei from lateral to medial position: 3 dpf (B), 20 dpf (C), and 2 mpf (D). Asterisk indicates the position of the eye. D. Schematic of DCP in adult zebrafish showing areas of spon1b:GFP expression (E and F) and depicting the habenula (Hb), its afferent projections within the stria medullaris (sm), its efferent projection: fasciculus retroflexus (FR), and target nuclei: interpeduncular nucleus (NIn), and superior raphe (SR). E-F. Para-sagittal cut through fresh-frozen adult Tg(spon1b-GFP) brain. GFP fluorescence highlights all DCP structures, including vHb nuclei, FR and sm (E). Also, cells of the bed nucleus of the stria medullaris (BNSM, arrow in E), and projections to NIn and SR in F. Rostral to the left. G. Schematics of the relative shape and position of the spon1b-positive nuclei of the Hb, as shown in H-I. H-L. Coronal sections immunostained for GFP: rostral (H) and caudal (I) Hb, NIn (J), and rostral (K) and caudal (L) SR. Scale bars: A-B: 100 μm; D: 25 μm; H-L: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP bu1Tg standard conditions Protruding-mouth ventral habenular nucleus IFL
Days 14-20 ventral habenular nucleus IFL
Days 45-89 ventral habenular nucleus IFL
Adult fasciculus retroflexus IFL
Adult habenula IHC
Adult superior raphe nucleus IHC
Adult superior raphe nucleus IFL
Adult ventral habenular nucleus IFL
Antibody Labeling Details No data available
Phenotype Details No data available
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