FIGURE

Fig. 5

ID

ZDB-FIG-120612-18

Publication

Lindeman et al., 2012 - Localized Products of futile cycle/ lrmp Promote Centrosome-Nucleus Attachment in the Zebrafish Zygote

Other Figures

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Fig. 5

C-Terminal Domains of Lrmp Facilitate Subcellular Targeting (A) Diagram of EFGP-fusion constructs. (B) Fusion construct RNAs encoding EGFP::LrmpCC-TM-L (top row), EGFP::LrmpTM-L (second row), and EGFP::LrmpCC construct (bottom two rows) were injected into one-cell WT embryos. Embryos expressing EGFP were fixed and processed for DAPI and anti-γ-tubulin immunostaining between 2.5 and 3.5 hpf. White boxes indicate fields shown at higher magnification (right). Scale bar represents 20 μm in all lower magnification panels. (C) Summary of results from EGFP::Lrmp C-terminal protein expression. Low viability (far right column) manifested as cell division defects and failure to undergo gastrulation, which led to embryo lysis. See also Figure S5.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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