FIGURE

Fig. 4

ID

ZDB-FIG-120525-22

Publication

Akagi et al., 2012 - Miniaturized Embryo Array for Automated Trapping, Immobilization and Microperfusion of Zebrafish Embryos

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Fig. 4

Assessment of microenvironmental conditions inside the chip:

A) 3D streamline of flow obtained by computational fluid dynamic simulations across the fully loaded device. Perfusion was simulated at a volumetric flow rate of 0.4 ml/min; B) Contours of shear stress (Pa) exerted on embryos across the whole device. There sections of the chip (upper, middle and lower part) are magnified for clarity. Perfusion was simulated at a volumetric flow rate of 0.4 ml/min; C) Performance curves of the chip as a function of the volumetric flow rate; D) Time-lapse images of developing zebrafish embryos collected every 24 hours. Embryos were loaded on a chip at the volumetric flow rate of 2 ml/min. Subsequently the chip was perfused at a rate of 0.4 ml/min for up to 72 hours.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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