Fig. 2

GFP and Sox21 expression in the zebrafish lens. (A, B) Stable transgenic lines expressing GFP in the lens under the control of Fugu CNE 6 (A) and Fugu CNE 17 (B) at 52 hpf. (C, D) sox21a and sox21b expression in zebrafish at the 20 somite stage. (E–H) Transient GFP-expression in the lens at 52 hpf activated by zebrafish CNE 6 (E), zebrafish CNE 17 (F) and zebrafish CNE 12 (G). In contrast to the zebrafish orthologue Fugu CNE 12 is not active in the lens (H). (I–L) GFP expression in stable transgenic lines. Only CNE 6 is active in the lens at 20 somites (I) whereas CNE 17 activity at this stage is limited to the CNS (J). At 30 hpf also CNE 17 is active in the lens (L) but in a more central domain than CNE 6 which seems to drive expression throughout the lens (K). (A, B, E–H) GFP fluorescence, anterior to the left. (C, D, I–L) Whole-mount in situ hybridizations, anterior to the left. Dorsal view (C, D, I, J), lateral view (K, L). Arrows indicate position of the lens during early stages. A dashed line marks the boundary of the lens in K and L. Tr (Takifugurubripes, Fugu), Dr (Danio rerio, zebrafish).