FIGURE

Fig. S2

ID

ZDB-FIG-120417-5

Publication

Tallafuss et al., 2012 - Turning gene function ON and OFF using sense and antisense photo-morpholinos in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. S2

Time course showing cleavage of the photo-element and in vivo time line. (A) Examples of mass spectroscopy reads obtained after in vitro UV exposure of gfp-AS-photo-MO at 0, 15 and 120 seconds, showing complete cleavage of the photo-element after 2 minutes. (B-I) In vivo time course showing onset of fluorescent protein expression in Tg(mnx1:Gal4-VP16,UAS:GFP) embryo exposed to 5 minutes UV using gal4-MO (C,G), gal4-AS-photo-MO (D,H) and gfp-AS-photo-MO (E,I). (C,D) No fluorescent signal in gal4-MO (C) and gal4-AS-photo-MO (D) injected embryos but signal in gfp-AS-photo-MO injected embryos (E) 1 hour after UV exposure. (H) Fluorescent signal in gal4-AS-photo-MO injected embryos 2 hours after UV exposure. Scale bar: 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development