Fig. S1

Western blots show proteins for EMSA and BiFC experiments. (A) Western blots for HAHer1, MycHer7, VSVGHer11, HisHer12, FlagHes6 and V5Her15. Expected sizes for EMSA constructs are: HAHer1, 38 kDa; MycHer7, 25 kDa; VSVGHer11, 32 kDa; HisHer12, 19 kDa; FlagHes6, 26 kDa; V5Her15, 20 kDa. All of the proteins run at about the expected size. Note that each protein synthesis reaction gives different levels of protein for the different Her constructs. (B) Western blot of TLF uninjected control and 250 ng RNA injections of Her1VN, Her7VN, Her11VN, Her12VN, Hes6VN and Her15VN. (C) Western blot of TLF uninjected control and 450 ng RNA injections of Her1VN, Her7VN, Her11VN, Her12VN, Hes6VN and Her15VN. Expected sizes for BiFC constructs are: Her1VN, 54 kDa; Her7VN, 41 kDa; Her11VN, 48kD; Her12VN, 35 kDa; Hes6VN, 42 kDa; Her15VN, 35 kDa. All of the proteins run at the expected size except Her12VN, which runs smaller and weaker. This result is consistent across several injections. Her11VN was never visualized on the blot despite exhibiting strong BiFC fluorescence at 400 ng/μl injections. The Multi Tag protein (abcam: ab36791) is a recombinant protein containing the epitope tags (GST, T7, HSV, c-myc, VSV, Glu-Glu, V5, E-tag, DYKDDDDK, S-tag, HA, 6×His) for use on western blots to estimate concentration of epitope-tagged proteins of interest.