FIGURE
Fig. 2
- ID
- ZDB-FIG-120316-52
- Publication
- Sapetto-Rebow et al., 2011 - Maternal topoisomerase II alpha, not topoisomerase II beta, enables embryonic development of zebrafish top2a-/- mutants
- Other Figures
- All Figure Page
- Back to All Figure Page
Fig. 2
blm arises from a mutation in the top2a gene. A) Mapping of blm reveals that the gene is located on chromosome 12. B) top2a was selected as a candidate gene and sequencing of mutant cDNA shows a point mutation (A→T) in Lys residue 335 resulting in a premature stop codon. C) A schematic of wildtype Top2a protein and the truncated proteins in hi3635, blm and can4 mutants D) Complementation assay performed to confirm that blm is top2a mutation. blm heterozygotes were crossed to hi3635 carriers and the resulting hybrid offspring present with blm phenotype. |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
| Fish: | |
|---|---|
| Observed In: | |
| Stage Range: | Long-pec to Protruding-mouth |
Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ BMC Dev. Biol.