Fig. 7

Semicircular canal formation in mif and iclp morphants. (A–O) phalloidin staining of epithelial pillars (ep), which form hubs of the developing SCC. (A–E) 3 dpf; (F–J) 4 dpf; (K–O) 5 dpf larvae. (A, F, K) control; (B, G, L) mif morphants; (C, H, M) mif morphants with capped mif RNAs; (D, I, N) iclp morphants; (E, J, O) iclp morphants with capped iclp RNAs. Anterior is to the left. Arrows: junction of the posterior bulge (pb) and posterior protrusion (pp). Arrowheads: junction between the ventral bulge (vb) and ventral protrusion (vp). Stars: junction of the anterior protrusion (ap) and the anterior bulge (ab). In mif morphants, a gap between the pb and the pp was observed, indicating fusion failure. Scale bar: 25 μm. n = 11 for the 3 dpf control, 12 for the 3 dpf mif morphants, 4 for the 3 dpf mif MOs + RNA. Among the 12 mif morphants, 9 had defects in SCC formation (75%). n for 4 dpf is 7 for the controls, 6 for the mif morphants, 6 for the mif MOs + RNA, 11 for the iclp morphants, 5 for the iclp MOs + RNA. Four out of 6 (67%) mif morphants had SCC defects. For 5 dpf larvae, n = 6 for control, 7 for mif morphants, 7 for mif MOs + RNA, 5 for iclp morphants, 6 for iclp MOs + RNA. Eighty six percent (6 out of 7) mif morphants had SCC defects.