FIGURE

Fig. 5

ID

ZDB-FIG-120207-3

Publication

Amir-Zilberstein et al., 2012 - Homeodomain protein otp and activity-dependent splicing modulate neuronal adaptation to stress

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Fig. 5

pCREB-Otp Protein Complex Is Recruited to crh and a2bp1 Promoters (A) A pool of 100 larvae per lane were subjected to stress challenges in bulk or left unchallenged. Larvae were lysed and subjected to IP with a resin-coupled anti-Otp antibody (Ab) followed by gel electrophoresis and protein electrotransfer to a membrane. Nitrocellulose filters were immunoblotted with an antibody directed to pCREB, stripped, and reblotted with an anti-Otp antibody. (B) Schematic representation of the sequential ChIP-reChIP procedure in which larvae lysates were first subjected to anti-Otp ChIP and the resulting Otp-DNA complex were eluted and subjected to a second ChIP with a pCREB antibody. (C) A pool of fish (300 larvae per lane) were subjected to stress challenges in bulk or left unchallenged followed by the ChIP-reChIP procedure using control IgG, anti-Otp, and anti-pCREB antibodies. The histograms show promoter co-occupancy analysis of Otp and pCREB (n = 2).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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