FIGURE

Fig. 5

ID

ZDB-FIG-120126-63

Publication

Tokarz et al., 2012 - Discovery of a novel enzyme mediating glucocorticoid catabolism in fish: 20beta-Hydroxysteroid dehydrogenase type 2

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Analysis of subcellular localization of zebrafish 20beta-HSD type 2 showed that the enzyme is localized in the endoplasmic reticulum. Pearson?s correlation coefficient for colocalization of 20beta-HSD type 2 and the endoplasmic reticulum marker is 0.84. The panels depict detection of recombinant 20beta-HSD type 2 with anti-myc FITC-labeled antibody (A), counterstaining of endoplasmic reticulum with DsRed (B), and nuclei with Hoechst 33342 dye (C), respectively, and the overlay of fluorescence patterns (D). Magnification: 40x.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Molecular and Cellular Endocrinology, 349(2), Tokarz, J., Mindnich, R., Norton, W., Möller, G., Hrabé de Angelis, M., and Adamski, J., Discovery of a novel enzyme mediating glucocorticoid catabolism in fish: 20beta-Hydroxysteroid dehydrogenase type 2, 202-13, Copyright (2012) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.