FIGURE

Fig. 5

ID

ZDB-FIG-111207-5

Publication

Ebert et al., 2005 - Calcium extrusion is critical for cardiac morphogenesis and rhythm in embryonic zebrafish hearts

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

NCX1h mutation leads to calcium overload in the tre ventricle. Analysis of intracellular calcium levels in 48 hpf embryos by calcium orange fluorescent dye. Selected sequential frames at H70-msec intervals are shown. (A–D) In wild-type embryos, Ca²⁺ entry into cardiomyocytes, denoted by dye, sweeps across the heart in a wave (brackets) correlated with the sequential contraction of the chambers. (E–H) In tre mutants, calcium orange levels in the ventricle remain static and appear elevated relative to the atrium, suggesting calcium overload in the ventricle. Calcium signals corresponding to one or a few adjoining cells briefly fluoresce in the fibrillating atrium (see faint signals to right of *). Image is anterior to the top. a, atrium; v, ventricle.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	slc8a1a^m139/m139 (AB)
Observed In:	heart
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA