FIGURE

Fig. S2

ID

ZDB-FIG-111129-14

Publication

Alexander et al., 2011 - Combinatorial roles for BMPs and Endothelin 1 in patterning the dorsal-ventral axis of the craniofacial skeleton

Other Figures

All Figure Page

Back to All Figure Page

Fig. S2

BMP signaling is sufficient and necessary for Tg(Bre:GFP) expression. (A-C) Whole-mount immunochemical staining for GFP protein at early gastrula stages, lateral views, ventral towards the left. (D-F) Whole-mount immunochemical staining for pSmad1/5/8 protein at early gastrula stages, lateral views, ventral towards the left. (A,D) Uninjected controls. (B,E) Embryos microinjected at the one-cell stage with 100 pg xBmp4 mRNA. (C,F) Embryos injected with 250 pg xBmp4. (G-I) Whole-mount immunochemical staining for GFP protein at 30 hpf, lateral views of the head, anterior towards the left. (G) Control embryo in which a DMSO-soaked bead (dashed line) was implanted behind the eye at 24 hpf. (H) Increased GFP surrounding a similarly implanted bead soaked in 10 µg/µl human recombinant BMP4/7. (I) Loss of GFP in an embryo implanted with a bead soaked in 500 μg/μl NOG. Abbreviations: cn, commissural neurons; D, dorsal; de, dorsal eye; m, margin; pa, pharyngeal arches; V, ventral.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development