FIGURE

Fig. 6

ID

ZDB-FIG-111128-84

Publication

Ben et al., 2011 - Targeted mutation of the talpid3 gene in zebrafish reveals its conserved requirement for ciliogenesis and Hedgehog signalling across the vertebrates

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Fig. 6

Disruption of primary ciliogenesis and aberrant localization of Hh signalling components in MZta3 embryos. (A-D) Confocal images of embryos fixed at the 10-somite (A,B) or 14-somite stage (C,D), and double stained with anti-acetylated tubulin (green) and anti-β tubulin (red) revealing the absence of cilia from the neural tube (B) and otic vesicle (D) of MZta3 mutants. (E,F) Similar preparations of embryos fixed at 24 hpf showing absence of long motile cilia from the pronephric duct of MZta3 mutants (F). (G-L) Localization of Hh pathway components in the primary cilia of somitic cells in 5- to 10-somite stage wild-type and MZta3 mutant embryos. Accumulation of a GFP-Dzip1 fusion protein in the basal body (G) is unaffected in MZta3 embryos (H). (I,K) GFP-tagged forms of Gli2a (I) and Kif7 (K) accumulate at the tip of primary cilia in wild-type cells; in the absence of the primary cilium in MZta3 embryos, both proteins shows a punctate localization in the basal body (J,L).

Expression Data

Antibodies:	Ab1-tuba Ab1-tubg1
Fish:	WT si:ch211-185a18.2^i264/+; si:ch211-185a18.2ⁱ²⁶⁵; si:ch211-185a18.2^i262/+
Anatomical Terms:	neural tube ciliary basal body neural tube cilium otic vesicle ciliary basal body otic vesicle cilium pronephric duct ciliary basal body pronephric duct motile cilium
Stage Range:	10-13 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	si:ch211-185a18.2^i264/+; si:ch211-185a18.2ⁱ²⁶⁵; si:ch211-185a18.2^i262/+
Observed In:	cilium assembly neural tube cilium otic vesicle cilium pronephric duct motile cilium
Stage Range:	10-13 somites to Prim-5

Phenotype Detail

Acknowledgments

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