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Fig. 4

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ZDB-FIG-111103-9

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Asaduzzaman et al., 2011 - Multiple cis-elements in the 5'-flanking region of embryonic/larval fast-type of the myosin heavy chain gene of torafugu, MYH(M743-2), function in the transcriptional regulation of its expression

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Fig. 4

EGFP expression was localized to both fast and slow muscle fibers in P2075-injected larvae and only fast muscle fibers in stable transgenic line (MYH_M743-2:EGFP) larvae. A–B: Fast muscle fibers expressing EGFP as reacted with F310 antibody in a P2075-injected larva at 3 dpf (A, lateral view; B, transverse section). C-D, Slow muscle fibers also expressing EGFP as reacted with F59 antibody (C, lateral view; D, transverse section) in a P2075-injected larva at 3 dpf. Some EGFP expressing muscle fibers were stained with F59 antibody (arrows in D). E-J: MYH_M743-2:EGFP transgene in a stable transgenic line larva showed EGFP expression in fast muscle fibers as reacted with F310 antibody (E, F, G; transverse sections) but not in slow muscle fibers as no EGFP expressing muscle fiber reacted with F59 antibody (H, I, J; transverse sections). Scale bars: 20 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Gene, 489(1), Asaduzzaman, M., Kinoshita, S., Siddique, B.S., Asakawa, S., and Watabe, S., Multiple cis-elements in the 5'-flanking region of embryonic/larval fast-type of the myosin heavy chain gene of torafugu, MYH(M743-2), function in the transcriptional regulation of its expression, 41-54, Copyright (2011) with permission from Elsevier. Full text @ Gene