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ZFIN ID: ZDB-FIG-111024-1
Matos-Cruz et al., 2011 - Unexpected diversity and photoperiod dependence of the zebrafish melanopsin system. PLoS One   6(9):e25111 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 5

Fig. 3

Diverse expression of zebrafish opn4-related genes in the developing retina.

Profile of opn4-related gene expression in the larval retina from 3 to 5 dpf. Following whole-mount RNA in situ hybridization, larvae were embedded in LR gold media and 4 μm sections prepared. In A, the lens and retinal cell layers are indicated (GCL, ganglion cell layer, INL, inner nuclear layer, PCL, photoreceptor cell layer). (A–C) opn4xa is expressed in a small subset of cells in the ganglion cell layer. (D–F) opn4xb is transcribed in bipolar cells in a broad domain of the INL. (G–I) opn4a is expressed in clusters of bipolar cells scattered throughout the INL. (J–L) opn4b transcripts are found in three domains within the INL, where amacrine (arrowhead in L), bipolar (arrow in K) and horizontal cells (open arrowhead in K) are located. (M–O) opn4.1 expression is weakly detected at 3 dpf but, one day later, strong expression is observed in horizontal cells in the outer shell of the INL. Sparse opn4.1 expression is also found in the photoreceptor cell layer.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
opn4.1 AB standard conditions Protruding-mouth retinal inner nuclear layer horizontal cell ISH
Day 4 retinal inner nuclear layer horizontal cell ISH
Day 5 retinal inner nuclear layer horizontal cell ISH
Day 5 retinal photoreceptor layer ISH
opn4a AB standard conditions Protruding-mouth retinal inner nuclear layer retinal bipolar neuron ISH
Day 4 retinal inner nuclear layer retinal bipolar neuron ISH
Day 5 retinal inner nuclear layer retinal bipolar neuron ISH
opn4b AB standard conditions Protruding-mouth retinal inner nuclear layer amacrine cell ISH
Protruding-mouth retinal inner nuclear layer horizontal cell ISH
Protruding-mouth retinal inner nuclear layer retinal bipolar neuron ISH
Day 4 retinal inner nuclear layer amacrine cell ISH
Day 4 retinal inner nuclear layer horizontal cell ISH
Day 4 retinal inner nuclear layer retinal bipolar neuron ISH
Day 5 retinal inner nuclear layer amacrine cell ISH
Day 5 retinal inner nuclear layer horizontal cell ISH
Day 5 retinal inner nuclear layer retinal bipolar neuron ISH
opn4xa AB standard conditions Protruding-mouth retinal ganglion cell layer ISH
Day 4 retinal ganglion cell layer ISH
Day 5 retinal ganglion cell layer ISH
opn4xb AB standard conditions Protruding-mouth retinal inner nuclear layer retinal bipolar neuron ISH
Day 4 retinal inner nuclear layer retinal bipolar neuron ISH
Day 5 retinal inner nuclear layer retinal bipolar neuron ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One