FIGURE

Fig. 2

ID
ZDB-FIG-111018-40
Publication
Liu et al., 2010 - A Zebrafish Cell Culture Assay for the Identification of MicroRNA Targets
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Fig. 2

ZSSJ and ZF cell lines express dicer1, ago1, and ago2. (A) Schematic showing the intron (line) and exon (box) organization of zebrafish dicer1, ago1, and ago2 and the location of primers (arrows) used for RT-PCR analysis. Primers were designed to amplify exons 6 and 9 of dicer1; 1 and 6 of ago1 and 1 and 4 of ago2. (B) RT-PCR analysis of dicer1, ago1, and ago2 expression in adult zebrafish tissues and (C) ZSSJ and ZF cell cultures. β-actin was used as a positive control and vasa as a gonad-specific control.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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