FIGURE

Fig. 2

ID

ZDB-FIG-111011-26

Publication

Fujimoto et al., 2011 - Gal80 intersectional regulation of cell-type specific expression in vertebrates

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Fig. 2

Gal4 drives strong expression in stable transgenic lines and can be inhibited by Gal80. Confocal maximum projections of 72 hours postfertilization (hpf) embryos; ventral views, anterior to the top. Embryos are heterozygous for the genotypes shown. Arrow points to ectopic green fluorescent protein (GFP) expression in eyes driven by Gal4-VP16 (A,D) not noted using native Gal4 (E). A?C: Live embryos imaged with identical laser power settings, showing that Gal4-driven expression (B) is stronger than a direct enhancer:GFP construct (C) and is comparable to Gal4-VP16-driven expression (A). D?F: Immunohistochemistry for GFP in fixed embryos again shows that Gal4-dependent expression (E) is comparable to Gal4-VP16 (D), but has minimal expression in other tissues (such as eye muscles, arrow). F: The original transgenic enhancer line Tg(otpb.A:egfp)^zc48 shows dimmer expression compared with Gal4-driven expression in E. G: A weakly expressing allele of elavl3:Gal80 only partially inhibits Gal4-dependent transgene expression. H: A strongly expressing elavl3:Gal80 allele completely inhibits Gal4-dependent expression. Insets for G and H show relative levels of Gal80 in situ expression. I: Gal80 is unable to inhibit Gal4-VP16-driven expression. Scale bar = 50μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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