FIGURE

Fig. 4

ID

ZDB-FIG-110909-62

Publication

Wang et al., 2011 - The regenerative capacity of zebrafish reverses cardiac failure caused by genetic cardiomyocyte depletion

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Effects of cardiomyocyte ablation on non-muscle cells. (A) Visualization of endocardial cells (green) and muscle (red) in sections from vehicle-or 4-HT-injected Z-CAT fish. The endocardial layer appears intact one week after massive cardiomyocyte loss. Single confocal slices are shown. (B) In situ hybridization for pu.1-expressing cells, which are prominent by 3-5 dpi. (C) Whole-mount visualization of epicardial nuclei in vehicle- or 4-HT-injected Z-CAT fish at 7 dpi, indicating higher epicardial cell density after myocardial injury. Inset shows enlarged view of boxed area. (D) Histological visualization of epicardial cells after myocardial ablation. Epicardial cells proliferate by 7 dpi and are incorporated into the regenerating myocardial wall by 14 dpi. Brackets indicate area containing tcf21-expressing epicardial cells. (E) Visualization of coronary vascular endothelial cells (green) in sections of the ventricular wall (outlined by dotted lines). Coronary vasculature is reduced in 4-HT-injected Z-CAT animals by 7 dpi, but restored by 14 dpi. (F) Sections from Z-CAT ventricles stained for the retinoic acid (RA)-synthesizing enzyme Raldh2. RA synthesis appears low in vehicle-injected tissue, but is strongly induced in epicardial (arrowheads) and endocardial (arrows) structures after cardiomyocyte ablation. Single confocal slices are shown. Scale bars: 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development