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Fig. S5

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ZDB-FIG-110812-13

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Paridaen et al., 2011 - The nucleolar GTP-binding proteins Gnl2 and nucleostemin are required for retinal neurogenesis in developing zebrafish

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Fig. S5

Gene expression changes upon Gnl2 LOF. (A and B) The earliest morphologically apparent phenotype of gnl2 mutants at 22 hpf, consisting of reduced forebrain (arrow and dashed lines), a thinner mid/hindbrain boundary (arrowhead) and smaller eyes. (C) Differentially expressed genes upon loss of Gnl2 at 22 hpf, categorized according to Gene Ontology (GO) function. A GO function was found for 23 out of the 44 total downregulated genes and 64 out of 96 total upregulated genes. (D–I) Confirmation of reduced expression of gnl2 (D and E; n = 9) and increased expression of the direct p53 transcriptional targets mdm2 (F and G; n = 6) and ccng1 (H and I; n = 7) in gnl2 mutants (E, G and I) as shown by in situ hybridization. All images are lateral view, anterior to the left. Scalebar 125 μm.

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Antibody Labeling

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Acknowledgments

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Reprinted from Developmental Biology, 355(2), Paridaen, J.T., Janson, E., Utami, K.H., Pereboom, T.C., Essers, P.B., van Rooijen, C., Zivkovic, D., and Macinnes, A.W., The nucleolar GTP-binding proteins Gnl2 and nucleostemin are required for retinal neurogenesis in developing zebrafish, 286-301, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.