Fig. 3
- ID
- ZDB-FIG-110811-14
- Publication
- Wright et al., 2011 - DeltaC and DeltaD interact as Notch ligands in the zebrafish segmentation clock
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Expression of DeltaC and DeltaD in retina and brain. (A-D) Sections of wild-type (WT) and mib mutant zebrafish retina at 24 hpf, immunostained with zdc2 and zdd2. (E,F) In situ hybridisation (ISH) of wild-type specimens (wholemounts) with probes detecting deltaC and deltaD mRNA. (G,H) Sections of hindbrain at 24 hpf, immunostained for DeltaC and DeltaD. (I-K) Section of retina and adjacent brain tissue in a 48 hpf embryo, doubly immunostained for DeltaC and DeltaD. In the retina the two proteins largely colocalise, but in the brain they are in distinct sets of cells. A-D and I-K are single confocal optical sections of 15 µm cryosections; G,H are projections of a small z-stack. A-D and G,H are counterstained for actin with fluorescent phalloidin (green). |