FIGURE

Fig. 2

ID

ZDB-FIG-110712-85

Publication

Boldajipour et al., 2011 - Cxcl12 evolution - subfunctionalization of a ligand through altered interaction with the chemokine receptor

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Fig. 2

In vivo functional analysis of PGC migration in Cxcl12 mutants. (A) Sequence alignment of zebrafish Cxcl12a and Cxcl12b. The signal peptide is marked in gray and residues that differ between the ligands are colored. Domain swaps in chimeric proteins used in C are indicated and the position of the analyzed point mutations highlighted in magenta. (B) Expression of a specific Cxcl12 form directed to the PGCs results in a local field of this protein that can interfere with the endogenous Cxcl12a gradient. Expression of an ineffective version of the ligand does not interfere with the migration of PGCs (left), whereas an effective ligand interferes with the gradient of the guidance cue (right). (C) The activity of chimeric and mutated Cxcl12 molecules in this assay. Bars show the mean percentage of ectopic PGCs per embryo. Error bars depict s.e.m. *, P<0.05 compared with control (ANOVA).

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

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