FIGURE

Fig. 5

ID

ZDB-FIG-110622-16

Publication

Alibaud et al., 2011 - A Mycobacterium marinum TesA mutant defective for major cell wall associated lipids is highly attenuated in Dictyostelium discoideum and zebrafish embryos

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Fig. 5

Infection of zebrafish with the Mma tesA::Tn mutant: CHT injections. A. Fish survival was scored for each of three sets of injected embryos (n = 24). P < 0.0001 for MmaM versus tesA::Tn and MmaM versus RD1. These results are representative of three independent experiments. B. Whole embryo bacterial counts of MmaM, RD1 or tesA::Tn-infected embryos. Results are expressed as mean cfu per fish � SEM from three independent experiments. Inoculates were MmaM (91 � 40), RD1 (172 � 130), tesA::Tn (144 � 16). At 7 dpi, P < 0.01 for MmaM versus tesA::Tn and P < 0.05 for MmaM versus RD1. The symbols used are the same as those presented in (A). C. Overlay of fluorescent and transmission images of individual infected fish at 5 dpi. Three independent fish injected with each strain are shown. Scale bar is 250 μm. Note the red autofluorescence of the yolk (Y). D. Live imaging of individual infected macrophages at 5 dpi in MmaM, RD1 and tesA::Tn embryos using fluorescence and DIC microscopy at high magnification (�600). The latter contain much lower bacterial loads.

Expression Data

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Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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