Fig. S7

Initiation and Maintenance of HSC Gene Expression are Unaffected in Dino Mutants a-f) Whereas initiation (a, b, d, e) and maintenance (c, f) of runx1 expression within the DA of dino mutants, chd^tt250 (Schulte-Merker et al., 1997), was not significantly affected in comparison to wild type embryos from the same in cross (red arrowheads), the expression of runx1 within the posterior ICM was substantially increased in dino mutants (blue arrows). g-j) gfi1 expression within the DA of dino mutants behaves in a similar manner to runx1 in that it also exhibits no significant differences from wild type expression (red arrowheads), but is ectopically expressed within the posterior ICM (blue arrowheads). k-l) Maintenance of arterial tbx20 is unaffected in the DA of dino mutants in comparison to wild type embryos from the same in cross (yellow arrowheads). The Bmp-dependent expression of tbx20 within the ventral cells under the yolk cell extension (Supp Fig 5, green arrowheads) is substantially increased in dino mutants compared to wild type embryos (orange arrowheads).