Fig. 3

CBX isoforms lacking the Pc box. (A) Schematic representation of zebrafish cbx4 and cbx6a transcripts and proteins. On the transcript cartoons, white parts indicate untranslated regions whereas red parts correspond to regions coding for the Pc boxes. On the protein drawings, the chromodomain is indicated as a blue triangle and the Pc box as a red circle. Amino acid length of the proteins is indicated. Primers used for the RT-PCR are shown as green arrows. (B) Detection of cbx6a1 and cbx6a2 transcripts in zebrafish larvae by RT-PCR. Total RNAs were extracted from 5 dpf zebrafish larvae and subjected to reverse transcription and PCR amplification using primers specific for cbx6a1 and cbx6a2, as indicated in (A), in presence (+ RT) or absence (- RT) of reverse transcriptase. The expected size of the PCR products is 135 and 167 bp for cbx6a1 and cbx6a2, respectively. (C) Schematic representation of human CBX2transcripts and proteins. White parts indicate untranslated regions whereas the Pc box coding region is shown in red on the CBX2 isoform 1 transcript. On the protein drawings, the chromodomain is indicated as a blue triangle and the Pc box as a red circle. Amino acid length of the proteins is indicated.