FIGURE

Fig. 8

ID

ZDB-FIG-110426-1

Publication

Schlombs et al., 2003 - Site-1 protease is required for cartilage development in zebrafish

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Fig. 8

Lipid defects in zebrafish s1p mutants. Oil red O staining of 48 hpf zebrafish larvae. (a and b) Lateral view. (c-f) Ventral view. goz (b and d) and sibling (a and c) larvae and larvae injected with 6 ng of s2p2 (e) and 12 ng of SCAP1 (f) PMOs are shown. Strong lipid staining can be observed around the eye (arrow), the otic vesicle (arrowhead), and in the heart (h) of sibling larvae (a), in addition to the region of developing trabeculae (arrow in c). Both sibling and goz larvae show a strong lipid staining of the yolk (asterisks in a and b). Lipid deposit is severely reduced in all other tissues in goz larvae (b), especially around the eye (arrow) and the otic vesicle (arrowhead). No lipid can be detected around the developing trabeculae in goz (arrow in d). (e and f) Injection of s2p and SCAP PMOs results in the same reduction of lipid. (Scale bars are 200 µm in a and b and 100 µm in c-f.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mbtps1^{tr6722/tr6722} (AB) WT + MO1-scap WT + MO2-mbtps2
Knockdown Reagents:	MO1-scap MO2-mbtps2
Observed In:	lipid localization
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA