ZFIN ID: ZDB-FIG-110426-1
Schlombs et al., 2003 - Site-1 protease is required for cartilage development in zebrafish. Proceedings of the National Academy of Sciences of the United States of America   100(24):14024-14029 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 8

Lipid defects in zebrafish s1p mutants. Oil red O staining of 48 hpf zebrafish larvae. (a and b) Lateral view. (c-f) Ventral view. goz (b and d) and sibling (a and c) larvae and larvae injected with 6 ng of s2p2 (e) and 12 ng of SCAP1 (f) PMOs are shown. Strong lipid staining can be observed around the eye (arrow), the otic vesicle (arrowhead), and in the heart (h) of sibling larvae (a), in addition to the region of developing trabeculae (arrow in c). Both sibling and goz larvae show a strong lipid staining of the yolk (asterisks in a and b). Lipid deposit is severely reduced in all other tissues in goz larvae (b), especially around the eye (arrow) and the otic vesicle (arrowhead). No lipid can be detected around the developing trabeculae in goz (arrow in d). (e and f) Injection of s2p and SCAP PMOs results in the same reduction of lipid. (Scale bars are 200 µm in a and b and 100 µm in c-f.)

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-scap standard conditions Long-pec lipid localization disrupted, abnormal
WT + MO2-mbtps2 standard conditions Long-pec lipid localization disrupted, abnormal
mbtps1tr6722/tr6722(AB) standard conditions Long-pec lipid localization disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA