FIGURE

Fig. 3

ID

ZDB-FIG-110207-36

Publication

Tehrani et al., 2011 - Antagonistic interactions of hedgehog, Bmp and retinoic acid signals control zebrafish endocrine pancreas development

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Elevated levels of Hh signaling impair RA-mediated induction of ectopic endocrine pancreas in the anterior endoderm. (A-O) Dorsal views (anterior to left) of pdx1 (A-C), insulin (D-F), neurod (G-I) and hhex (J-L) expression at 24 hpf and ptf1a (M-O) expression at 48 hpf, comparing embryos transiently treated from 9-10 hpf with DMSO (A,D,G,J,M), retinoic acid (RA; 1 μM) (B,E,H,K,N) or RA plus purmorphamine (25 μM) (C,F,I,L,O). Brackets indicate ectopic expression and arrowheads indicate endogenous expression. The number of embryos displaying similar expression patterns is indicated in each case. (P) qPCR of pdx1 expression at 24 hpf in DMSO-, RA- and RA plus purmorphamine-treated embryos (error bars show s.e.m.). Asterisks indicate a significant change (at least 2 s.d.) compared with control.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development