FIGURE

Fig. S1

ID

ZDB-FIG-110111-20

Publication

Feng et al., 2010 - Live Imaging of Innate Immune Cell Sensing of Transformed Cells in Zebrafish Larvae: Parallels between Tumor Initiation and Wound Inflammation

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Fig. S1

V12RAS induces pro-inflammatory gene up-regulation in zebrafish larvae. (A) RT-PCR showing up-regulation of pro-inflammatory genes in V12RAS⁺ larvae at 4dpf compared with their V12RAS^- siblings. (B) qPCR showing increased expression of il1² and cxcl1 in 5-dpf hsp:V12RASeGFP larvae compared with WT after both have been heat shocked for 6 h. (C) Fluorescent in situ hybridization of arginase1 (cyan) combined with L-plastin antibody staining for leukocytes (magenta) and anti-RAS antibody staining for V12RAS⁺ cells (green) in 7-dpf V12RAS⁺ larvae. (D) Anti-TNFα antibody staining (cyan) combined with anti-L-plastin antibody staining for leukocytes (magenta) in 7-dpf larvae with V12RASeGFP⁺ clones (green)-arrowheads indicate TNFα signal inside some of the L-plastin⁺ cells. *, p<0.05. Scale bars = 20 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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