FIGURE

Fig. 4

ID

ZDB-FIG-110105-39

Publication

Hans et al., 2011 - Generation of a non-leaky heat shock-inducible Cre line for conditional Cre/lox strategies in zebrafish

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Fig. 4

Cre-mediated recombination of the (hsp70l:mCherry-T2A-CreER^T2)^#152 allele in the red-to-green reporter line in the absence and presence of heat and TAM. A: Scheme of the transgene combination of the embryos depicted in B?E. B: In the absence of heat and TAM, the combination of the Tg(hsp70l:mCherry-T2A-CreER^T2)^#15 allele with the red-to-green reporter line results in strong DsRed2 but no EGFP expression in double transgenic embryos. C: Application of TAM leads to EGFP expression in the Tg(hsp70l:mCherry-T2A-CreER^T2)^#15 enhancer trap as well as a mosaic pattern in double transgenic embryos at permissive temperatures. D: In the absence of TAM, mosaic EGFP expression can be detected after heat treatment. E: Application of TAM and heat results in strong ubiquitous EGFP expression in double transgenic embryos. B?E: Lateral views of live 24-hpf embryos.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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