Fig. 7

Axon fragmentation following spontaneous pruning and cell death was delayed in Wld^S-expressing neurons. Time-lapse confocal image stack projections of WT (A-H) or Wld^S (I-P) neurons. (A-D) 40 hpf wild-type pruning beginning at –2 hours (A, before pruning). White arrow in B indicates the site of detachment of a spontaneously pruned branch (0 hours). Red arrows in C and D indicate fragmentation and clearance, respectively, of the pruned axon (see Movie 7 in the supplementary material). (E-H) 32 hpf wild-type spontaneous death example, beginning at –2 h (E, before apoptosis). White arrowheads in F and G indicate a dying cell body. Red arrow in H indicates axon fragmentation resulting from cell death (see Movie 8 in the supplementary material). (I-L) 48 hpf Wld^S pruning beginning with –1 hour (I, before pruning). White arrow in J indicates site of detachment of spontaneously pruned branch (0 h). Red arrowheads in K and L indicate a pruned branch that persists without degenerating (see Movie 9 in the supplementary material). (M-P) 36 hpf Wld^S cell death example beginning at –2.5 hours (M, during apoptosis). White arrowheads in M and N indicate a dying cell body. White arrow in N indicates a point of axon separation (0 hours). Red arrow in O indicates degeneration of the proximal axon nearest to the dead cell body. Red arrowhead in P indicates a large distal axon fragment that has remained intact long after cell death and proximal axon degeneration (see Movie 10 in the supplementary material). Scale bars: 50 μm.