FIGURE

Fig. S5

ID

ZDB-FIG-101129-2

Publication

Chung et al., 2010 - Suppression of Alk8-mediated Bmp signaling cell-autonomously induces pancreatic β-cells in zebrafish

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Fig. S5

alk8 splice-blocking morpholino-injected donor cells behave like control cells and can give rise to dorsal bud-derived endocrine cells. (A) Schematic diagram of the cell transplantation protocol. Tg(ins:GFP) donors were injected with cas/sox32 mRNA and alk8 MO along with rhodamine dextran, and the cells were transplanted into Tg(ins:GFP) hosts. (B and C) Ventral confocal images of Tg(ins:GFP) (green), β-catenin (white), and rhodamine dextran (red) at 18 hpf (the notochord is outlined by yellow dashed lines). The donor cells (red) did not give rise to ectopic Tg(ins:GFP)-expressing cells, because maternally derived Alk8 is present during early stages.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA