Fig. 1

PGRN knockdown results in reduced motor axon outgrowth.

A) Knockdown of grna, by morpholino targeted to both the start codon (ATG) and 5U′UTR region of zebrafish PGRN sequence, produced a dose dependent decrease in axonal length compared to mismatch Control MO injected embryos.■Significantly different from 200 μM Control MO, p<0.036; # significantly different from 50 μM MO, p<0.001; grna CO-MO (ATG): n = 41; grna CO-MO (UTR): n = 20; grna ATG-MO, 50 μM: n = 40, 100 μM: n = 40, 200 μM: n = 41; grna 5′UTR-MO, 50 μM: n = 27, 100 μM: n = 28, 200 μM: n = 14; Knockdown of grnb produced a similar, but more subtle, axonal shortening. * Significantly different from 600 μM Control MO, p<0.038; # significantly different from 200 μM MO, p<0.05; grnb CO-MO (ATG): n = 27; grnb CO-MO (UTR): n = 10; grnb ATG-MO, 200 μM: n = 40, 400 μM: n = 36, 600 μM: n = 41; grnb 5′UTR-MO, 200 μM: n = 9, 400 μM: n = 12, 600 μM: n = 12; B) The two MO used simultaneously had a cumulative effect; * significantly different from Control MO a + b, p<0.002; # significantly different from all other groups p<0.0001. Buffer injected: n = 20, CO-MO (A + B): n = 34, grnb MO: n = 36, grna MO: n = 36, grna + grnb MO: n = 36. All bars represent mean ± s.e.m.