FIGURE

Fig. 4

ID

ZDB-FIG-101119-9

Publication

Distel et al., 2009 - Optimized Gal4 genetics for permanent gene expression mapping in zebrafish

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Fig. 4

Rh3/5-Kalooping identifies the secondary octaval nucleus (SON) as rhombomere 5-derived. Immunhistochemistry for GFP (green) and Cholinacetyltransferase (ChAT; red) on brain vibratome sections of adult zebrafish derived from crosses of (A) rh3/5:KalTA4 × 4xKGFP and (B–F) rh3/5:KalTA4 × 4xKaloop. All nuclei were counterstained with DAPI (blue). (A and B) Sagittal sections of the hindbrain. (C) Transverse section through the caudal GFP-expressing hindbrain region (r5) in B. (midline marked by dashed line). (D) Sagittal section (rostral is left) showing GFP-positive dendrites in the crista cerebellaris (white arrow). (E) Axons of GFP-positive neurons project through the midbrain into the (F) torus semicircularis. Note typical periventricular cholinergic cells in optic tectum. Abbr.: cb: corpus cerebelli, cc: crista cerebellaris, llf: lateral longitudinal fascicle, nVI: rostral and caudal abducens nuclei, OEN: octavolateralis efferent neurons, r: rhombomere, SGVN: secondary gustatory/viscerosensory nucleus, ts: torus semicircularis, tec: optic tectum, va: valvula cerebelli.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA