Fig. S2

Hypoxia promotes LLC tumor cell invasion, dissemination and metastasis. (A and D) DiI-labeled LLC-vector tumor cells were injected into the perivitelline space of 48 h post-fertilization embryos and tumor cell invasion, dissemination, and metastasis were detected under normoxia and hypoxia using fluorescent microscopy at day 3 post-injection. Arrows in A and D indicate primary tumors. Arrowheads indicate disseminated tumor foci. (Scale bar, 500 μm.) (B and E) High-resolution micrographs of A and D, respectively. (Scale bar,100 μm.) (C and F) 3-D micrographs of confocal images of tumors (red) and tumoral as well as peritumoral vasculatures (green). (Scale bar, 10 μm.) (G) Quantification of tumor volume (n = 11/group). (H) Quantification of numbers of disseminated tumor foci (n = 11/group). (I) Quantification of maximal distances of metastasis (n = 11/group). (J) Quantification of tumor vessel density relative to tumor sizes (n = 7/group). Data are represented as mean ± SEM.