FIGURE

Fig. 5

ID

ZDB-FIG-101111-45

Publication

Prykhozhij, 2010 - In the Absence of Sonic Hedgehog, p53 Induces Apoptosis and Inhibits Retinal Cell Proliferation, Cell-Cycle Exit and Differentiation in Zebrafish

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Fig. 5

Wild-type and shh^-/- embryos at 24hpf (A-D) and 56 hpf (E, F) carrying p53 reporter transgene (PG13p21::nlsEGFP) were transversally sectioned, nuclei were stained with DAPI and apoptotic cells - with TUNEL, nlsEGFP signal is endogeneous (A-F). The developing nervous system of shh^-/- mutant embryos showed high level of p53 reporter expression and apoptosis compared to wild-type embryos at both 24 (B-D) and 56 hpf (F). (A) At 24 hpf, p53 reporter was strongly induced in the shh^-/- mutant compared to the wild-type retina, but there was no elevated apoptosis in the shh^-/- mutant retina. (B) Expression of p53 reporter and TUNEL labeling in midbrain of shh^-/- mutant and wild-type embryos at 24 hpf. (C) Expression of p53 reporter and TUNEL labeling in hindbrain of shh^-/- mutant and wild-type embryos at 24 hpf. (D) Expression of p53 reporter and TUNEL labelling in spinal cord of shh^-/- and wild-type at 24 hpf. (E) At 56 hpf, both p53 reporter expression and apoptosis were much higher in the shh^-/- retina than in the wild-type retina. (F) Expression of p53 reporter and TUNEL labelling in hindbrain regions of shh^-/- and wild-type embryos at 56 hpf.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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