ZFIN ID: ZDB-FIG-100924-6
Wen et al., 2010 - Distinct roles for two synaptotagmin isoforms in synchronous and asynchronous transmitter release at zebrafish neuromuscular junction. Proceedings of the National Academy of Sciences of the United States of America   107(31):13906-13911 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 4
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig. 3 Effects of syt2 knockdown on release. (A) The 10-s train of EPCs in response to 100-Hz stimulation for a syt2 morpholino fish. Two regions are expanded on a fast time scale corresponding to times when, in wild-type fish, release is either largely synchronous (A1) or asynchronous (A2). Asterisks indicate EPCs considered to be synchronous. The increased frequency of synchronous events in A2 is likely due to the heightened probability that an asynchronous event will fall within the window defined for synchronous detection. (B) Comparison of the contributions of asynchronous (blue triangles) versus synchronous (red circles) to total release (black squares) over the time course of 10 s of stimulation. (C) SV2 presynaptic label (green) and postsynaptic α-bungarotoxin (α-btx) label (red) in wild type (Upper Left) and syt2 morpholino (Upper Right) fish. Znp-1 antibody labeled syt2 protein (green) colocalizes with α-btx (red) label in wild-type fish (Lower Left) but is absent in syt2 morpholino fish (Lower Right). (Scale bar, 10 μm.) (D) Example traces of spontaneous synaptic currents from wild type (Top) and syt2 morpholino (Bottom) fish. A bar graph of spontaneous event frequency for wild-type fish (392 ± 263; n = 6 recordings), syt2 morpholino fish (18 ± 14; n = 10 recordings), and syt7 morpholino fish (17 ± 21; n = 6 recordings). ***P < 0.001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
chrna1 WT standard conditions Protruding-mouth to Day 4 skeletal muscle cell acetylcholine-gated channel complex OTHR
WT + MO1-syt2b standard conditions Protruding-mouth to Day 4 skeletal muscle cell acetylcholine-gated channel complex OTHR
syt2b znp-1 WT standard conditions Protruding-mouth to Day 4 motor neuron axon terminus IHC
znp-1 WT + MO1-syt2b standard conditions Protruding-mouth to Day 4 Not Detected motor neuron axon terminus IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-SV2 IHC WT standard conditions Protruding-mouth - Day 4 motor neuron axon terminus
IHC WT + MO1-syt2b standard conditions Protruding-mouth - Day 4 motor neuron axon terminus
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-syt2b standard conditions Protruding-mouth - Day 4 motor neuron axon terminus physical object quality, abnormal
Protruding-mouth - Day 4 synchronous neurotransmitter secretion decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA