FIGURE

Fig. 6

ID

ZDB-FIG-100809-11

Publication

Rodriguez-Mari et al., 2010 - Sex Reversal in Zebrafish fancl Mutants Is Caused by Tp53-Mediated Germ Cell Apoptosis

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Fig. 6

Increased germ cell apoptosis in fancl mutants at 25 dpf.

Immunodetection of apoptosis by anti-active Caspase-3 in paraffin sections of gonads of wild-type sibling controls (WT) and fancl homozygous mutants (fancl-/-) at 25 dpf (A,B). Presence of Caspase-3-positive cells (shown in red) was lower in gonads of WT (A) than in fancl mutants (B). Gonads outlined by a dashed line (A,B). Bar graph representing the average number of Caspase-3-positive germ cells in each genotype: wild-type sibling controls (WT; n = 6) and fancl homozygous mutants (fancl-/-; n = 6) at 25 dpf (C). Results showed that the average number of apoptotic germ cells in fancl mutants (x- = 99±43) was about three fold higher than in wild-type sibling controls (x- = 35±14), revealing an abnormal increase of germ cell apoptosis in fancl mutants at 25 dpf, a critical period for sex determination (C).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fancl^{nkhg10aEt/nkhg10aEt}
Observed In:	primordial germ cell
Stage:	Days 21-29

Phenotype Detail

Acknowledgments

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