Fig. 2

copeb is required for embryonic development. Live copeb splice donor morphants and copeb ATG morphants were examined on 1–4 dpf. (a) Live images of Tg(fabp10-RFP) embryos showed copeb slice donor morphants exhibit pericardial, yolk sac and cephalic edema at 48 and 72 hpf and a small for size liver. All images are taken at the same magnification; scale bar = 100 μm. (b) Live images of embryos showed a range of phenotypes of copeb ATG morpholino-injected embryos: normal (resembling embryos injected with the control morpholino (top), moderately affected embryos with pericardial edema, unconsumed yolk (Y) and blood congestion (middle 2 embryos) and severely affected, runted embryos (bottom). Bottom panels show the pericardial edema (PE; black circle) in morphants. These characteristics were observed in at least 10 clutches of copeb ATG morphants. (c) Quantification of the percent of embryos displaying the morphant phenotype at 72 and 96 hpf demonstrate that the phenotype becomes more severe as a function of morpholino concentration and time. Values represent the average of four clutches of at least 100 embryos were scored for each clutch on each day. Error bars indicate the standard deviation of the mean. (d) copeb splice donor morphants were quantified by phenotype and compared to uninjected controls at 96 hpf. Data are the average of at least 50 embryos per group in 3 clutches. Error bars indicate the standard deviation of the mean.